Frzb-1 is a secreted antagonist of Wnt signaling expressed in the Spemann organizer

Cell. 1997 Mar 21;88(6):747-56. doi: 10.1016/s0092-8674(00)81921-2.

Abstract

Frzb-1 is a secreted protein containing a domain similar to the putative Wnt-binding region of the frizzled family of transmembrane receptors. Frzb-1 is widely expressed in adult mammalian tissues. In the Xenopus gastrula, it is expressed and regulated as a typical Spemann organizer component. Injection of frzb-1 mRNA blocks expression of XMyoD mRNA and leads to embryos with enlarged heads and shortened trunks. Frzb-1 antagonizes the effects of Xwnt-8 ectopic expression in a non-cell-autonomous manner. Cultured cells transfected with a membrane-tethered form of Wnt-1 bind epitope-tagged Frzb-1 in the 10(-10) M range. The results strengthen the view that the Spemann organizer is a source of secreted inhibitory factors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Communication / physiology
  • Cells, Cultured / physiology
  • Embryonic Induction / physiology
  • Gastrula / physiology
  • Glycoproteins*
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Kidney / cytology
  • Mammals
  • Mice
  • Mitogens / antagonists & inhibitors*
  • Mitogens / genetics
  • Mitogens / metabolism
  • Molecular Sequence Data
  • Muscle, Skeletal / embryology
  • Protein Binding / physiology
  • Proteins / antagonists & inhibitors*
  • Proteins / genetics
  • Proteins / metabolism
  • Proteins / physiology*
  • Proto-Oncogene Proteins / antagonists & inhibitors*
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / metabolism
  • RNA, Messenger / metabolism
  • Sequence Homology, Amino Acid
  • Wnt Proteins
  • Wnt1 Protein
  • Xenopus / embryology*
  • Xenopus Proteins
  • Zebrafish Proteins*

Substances

  • Glycoproteins
  • Intracellular Signaling Peptides and Proteins
  • Mitogens
  • Proteins
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • WD repeat containing planar cell polarity effector
  • WNT1 protein, Xenopus
  • WNT1 protein, human
  • Wnt Proteins
  • Wnt1 Protein
  • Wnt1 protein, mouse
  • Xenopus Proteins
  • Zebrafish Proteins
  • wnt8a protein, Xenopus

Associated data

  • GENBANK/U68057
  • GENBANK/U68058
  • GENBANK/U68059