TLP1: a gene encoding a protein component of mammalian telomerase is a novel member of WD repeats family

Cell. 1997 Mar 21;88(6):875-84. doi: 10.1016/s0092-8674(00)81933-9.

Abstract

We have cloned and characterized the rat telomerase protein component 1 gene (TLP1), which is related to the gene for Tetrahymena p80. The cDNA encodes a 2629 amino acid sequence and produces the TLP1 proteins p240 and p230. The anti-TLP1 antibody specifically immunoprecipitated the telomerase activity. Moreover, p240 and p230 were copurified with telomerase activity in a series of extensive purification experiments. These results strongly suggest that the TLP1 proteins are components of, or are closely associated with, the rat telomerase. A pulse-chase experiment showed that p240 is modified to p230 in vivo. p230 was the dominant form in telomerase-positive cells, suggesting that modification of the TLP1 protein may regulate telomerase activity in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Blotting, Western
  • COS Cells / physiology
  • Cloning, Molecular
  • DNA, Complementary / analysis
  • Gene Expression Regulation, Enzymologic / physiology
  • Humans
  • Mammals
  • Mice
  • Molecular Sequence Data
  • Precipitin Tests
  • Rats
  • Repetitive Sequences, Nucleic Acid*
  • Sequence Homology, Amino Acid
  • Telomerase / genetics*

Substances

  • DNA, Complementary
  • Telomerase

Associated data

  • GENBANK/U89282