Cloning, sequencing and expression of VAT, a CDC48/p97 ATPase homologue from the archaeon Thermoplasma acidophilum

FEBS Lett. 1997 Mar 10;404(2-3):263-8. doi: 10.1016/s0014-5793(97)00138-5.


A member of the AAA family of Mg2(+)-ATPases from the archaeon Thermoplasma acidophilum has been cloned and expressed in Escherichia coli. The protein, VCP-like ATPase of Thermoplasma acidophilum (VAT), is a homologue of SAV from Sulfolobus acidocaldarius and CdcH of Halobacterium salinarium, and belongs to the CDC48/VCP/p97 subfamily. The deduced product of the vat gene is 745 residues long (Mr 83,000), which has an optimal Mg2(+)-ATPase activity at 70 degrees C. Electron microscopy shows the purified protein to form single and double homo-hexameric rings. Although the symmetry is different, the appearance of the complexes formed of two rings resembles the 20S proteasome and Hsp60/GroEL.

Publication types

  • Comparative Study

MeSH terms

  • Adenosine Triphosphatases / biosynthesis*
  • Adenosine Triphosphatases / chemistry
  • Adenosine Triphosphatases / ultrastructure
  • Amino Acid Sequence
  • Archaeal Proteins
  • Ca(2+) Mg(2+)-ATPase / biosynthesis*
  • Ca(2+) Mg(2+)-ATPase / chemistry
  • Ca(2+) Mg(2+)-ATPase / ultrastructure
  • Cell Cycle Proteins / chemistry
  • Cloning, Molecular
  • Escherichia coli
  • Genes, Bacterial
  • Halobacterium / enzymology
  • Microscopy, Electron
  • Molecular Sequence Data
  • Molecular Weight
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / ultrastructure
  • Sequence Homology, Amino Acid
  • Sulfolobus / enzymology
  • Thermoplasma / enzymology*
  • Thermoplasma / genetics
  • Valosin Containing Protein


  • Archaeal Proteins
  • Cell Cycle Proteins
  • Recombinant Proteins
  • Adenosine Triphosphatases
  • Ca(2+) Mg(2+)-ATPase
  • Valosin Containing Protein
  • vat protein, Thermoplasma acidophilum

Associated data

  • GENBANK/U78072