Construction and characterization of the soybean leaf metalloproteinase cDNA

FEBS Lett. 1997 Mar 10;404(2-3):283-8. doi: 10.1016/s0014-5793(97)00141-5.

Abstract

The cloning and analysis of a cDNA clone encoding the soybean metalloproteinase obtained by polymerase chain reaction (PCR) and the rapid amplification of cDNA ends (RACE) reaction are described. The cDNA was constructed from poly(A)+ RNA isolated from 15-17 day old leaves. The deduced amino acid sequence of the cDNA reveals that the plant metalloproteinase is synthesized as a preproenzyme and the proenzyme form shares a structural motif, responsible for maintenance of inactive zymogen, with the matrix metalloproteinase (e.g. collagenase) family of enzymes from vertebrate origin. Northern and Western blot analysis demonstrated that the metalloproteinase transcript and protein are under a strict developmental program in that both are expressed only in leaf tissue and in a temporal fashion. The physiological function of the metalloproteinase still remains unclear although the data suggest that the enzyme is extracellular and a portion of the mature form of the enzyme is tightly bound to the cell wall.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA, Complementary
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Plant
  • Humans
  • Metalloendopeptidases / biosynthesis*
  • Metalloendopeptidases / chemistry*
  • Metalloendopeptidases / isolation & purification
  • Molecular Sequence Data
  • Plant Leaves
  • Polymerase Chain Reaction
  • RNA, Messenger / biosynthesis
  • Random Amplified Polymorphic DNA Technique
  • Sequence Homology, Amino Acid
  • Soybeans / enzymology*
  • Transcription, Genetic

Substances

  • DNA, Complementary
  • RNA, Messenger
  • Metalloendopeptidases

Associated data

  • GENBANK/AF062640