Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation

FEBS Lett. 1997 Mar 10;404(2-3):319-23. doi: 10.1016/s0014-5793(97)00014-8.


We investigated the role of Sonic hedgehog (SHH) in osteoblast differentiation and bone formation. The numbers of ALP-positive cells in the mouse fibroblastic cell line C3H10T1/2 and the mouse osteoblastic cell line MC3T3-E1 were increased by co-culture with chicken fibroblasts transfected with chicken Shh cDNA encoding amino-terminal peptide (Shh-N). The conditioned medium of Shh-N-RCAS-transfected chicken fibroblast cultures also significantly increased ALP activity in both C3H10T1/2 and MC3T3-E1 cells. Intramuscular transplantation of Shh-N-RCAS-transfected chicken fibroblasts into athymic mice induced ectopic bone formation. These results indicate that SHH induces osteoblast differentiation and ectopic bone formation.

MeSH terms

  • 3T3 Cells
  • Animals
  • Bone Development*
  • Cell Differentiation
  • Cell Line
  • Chick Embryo
  • Coculture Techniques
  • Embryonic Induction
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • Fibroblasts / transplantation
  • Hedgehog Proteins
  • Kinetics
  • Mice
  • Mice, Inbred C3H
  • Mice, Nude
  • Osteoblasts / cytology*
  • Osteoblasts / transplantation
  • Peptide Fragments / biosynthesis
  • Protein Biosynthesis*
  • Recombinant Proteins / biosynthesis
  • Time Factors
  • Trans-Activators*
  • Transfection


  • Hedgehog Proteins
  • Peptide Fragments
  • Recombinant Proteins
  • Trans-Activators