Survival of Vi-capsulated and Vi-deleted Salmonella Typhi Strains in Cultured Macrophage Expressing Different Levels of CD14 Antigen

FEMS Microbiol Lett. 1997 Feb 15;147(2):259-65. doi: 10.1111/j.1574-6968.1997.tb10251.x.


We examined the intracellular survival of Vi-capsulated (lipopolysaccharide; (LPS)-masked) and Vi-deleted (LPS-exposed) Salmonella typhi strains inside macrophage cell lines. Growth of LPS-exposed S. typhi was inhibited in both mouse and human macrophage cell lines. However, the LPS-exposed strain survived in a CD14-deficient mouse macrophage cell lines. Wild-type S. typhi strain, which expressed the Vi antigen and masked LPS, survived in the resting human macrophage cell line. When the Vi-capsulated S. typhi entered the cells, the production of tumor necrosis factor-alpha (TNF-alpha) was suppressed. In contrast, S. typhimurium and LPS-exposed S. typhi stimulated the macrophages to produce a high level of TNF-alpha.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antigens, Bacterial / drug effects
  • Antigens, Bacterial / immunology
  • Bacterial Capsules / drug effects*
  • Bacterial Capsules / immunology
  • Cells, Cultured
  • Humans
  • Immunohistochemistry
  • Lipopolysaccharide Receptors / immunology*
  • Lipopolysaccharides / pharmacology
  • Macrophage Activation
  • Macrophages / immunology*
  • Macrophages / metabolism
  • Macrophages / microbiology
  • Mice
  • Phagocytosis / drug effects
  • Phagocytosis / immunology
  • Salmonella typhi / drug effects*
  • Salmonella typhi / immunology
  • Salmonella typhi / pathogenicity*
  • Tumor Necrosis Factor-alpha / biosynthesis
  • Typhoid Fever / immunology*


  • Antibodies, Monoclonal
  • Antigens, Bacterial
  • Lipopolysaccharide Receptors
  • Lipopolysaccharides
  • Tumor Necrosis Factor-alpha