Immunological Characterization of a Neuronal Antibody (anti-Tr) Associated With Paraneoplastic Cerebellar Degeneration and Hodgkin's Disease

J Neuroimmunol. 1997 Apr;74(1-2):55-61. doi: 10.1016/s0165-5728(96)00205-6.

Abstract

We studied an autoantibody (called anti-Tr), found in the serum and CSF of five patients with paraneoplastic cerebellar degeneration (PCD) and Hodgkin's disease (HD). Anti-Tr antibodies labelled the cytoplasm of Purkinje cells of human and rat cerebellum. The molecular layer of rat cerebellum showed a characteristic dotted pattern suggestive of immunoreactivity of dendritic spines of Purkinje cells. Patients with cerebellar disorders without HD (159) or HD without PCD (30) did not harbor anti-Tr antibodies. Immunoblots of human Purkinje cells or rat and mouse cerebellum were negative. Anti-Tr antibodies, as defined in this study, appear specific for HD-associated PCD. The immunohistochemical pattern described in the rat cerebellum coupled with the absence of reactivity in the immunoblot may be used to identify anti-Tr antibodies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Animals
  • Antibodies / analysis
  • Antibodies / cerebrospinal fluid
  • Antibodies / immunology*
  • Cerebellar Diseases / complications
  • Cerebellar Diseases / immunology*
  • Female
  • Hodgkin Disease / cerebrospinal fluid
  • Hodgkin Disease / complications
  • Hodgkin Disease / immunology*
  • Humans
  • Immunoblotting
  • Immunohistochemistry
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Nerve Degeneration*
  • Neurons / immunology*
  • Paraneoplastic Syndromes / cerebrospinal fluid
  • Paraneoplastic Syndromes / complications
  • Paraneoplastic Syndromes / immunology*
  • Rats
  • Rats, Wistar
  • Tissue Distribution

Substances

  • Antibodies