A total of 453 bulls belonging to 11 half-sib families of Finnish Ayrshires were genotyped for six microsatellite markers on chromosome 9. The data were used in an attempt to map quantitative trait loci applying regression as a multimarker approach. For association analysis with a granddaughter design, the EBV for 12 traits were used: milk yield, protein yield, fat percentage, protein percentage, daughter weight, bull growth, calf mortality, days open, fertility treatments, nonreturn rate, SCC, and clinical mastitis. The empirical values of significance thresholds were determined using a permutation test on the experimental data. Although no significant effects were found, the results indicate some support for the existence of a locus on chromosome 9 that affects milk and protein yields.