Regulation of aquaporin-2 Gene Transcription by GATA-3. Off

Biochem Biophys Res Commun. 1997 Mar 6;232(1):65-8. doi: 10.1006/bbrc.1997.6236.

Abstract

To evaluate the functional role of GATA motifs in the 5'-flanking region of a kidney-specific AQP-2 water channel gene, we sought to isolate a GATA factor(s) expressed in collecting ducts and determined the role on the AQP-2 promoter. Two cDNAs encoding GATA factors were isolated from rat kidney, whose sequences were highly homologous with human GATA-2 and -3. Reverse-transcription PCR using dissected nephron segments revealed that rat GATA-3 but not GATA-2 was expressed in collecting ducts, thus indicating that GATA-3 could interact with GATA motifs in the AQP-2 promoter. Transactivation experiments utilizing the rat GATA-3 expression vector indicated that rat GATA-3 increased the AQP-2 promoter activity about fourfold. These results indicated that GATA motifs in the 5'-flanking region of the hAQP-2 gene were functional cis-elements and that GATA-3 in collecting ducts may be one of the important regulators of AQP-2 expression in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aquaporin 2
  • Aquaporin 6
  • Aquaporins*
  • Cloning, Molecular
  • DNA, Complementary
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / physiology*
  • GATA2 Transcription Factor
  • GATA3 Transcription Factor
  • Humans
  • Ion Channels / genetics*
  • Nephrons / metabolism
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Rats
  • Trans-Activators / genetics
  • Trans-Activators / physiology*
  • Transcription Factors / genetics
  • Transcription, Genetic / physiology*

Substances

  • AQP2 protein, human
  • Aqp2 protein, rat
  • Aquaporin 2
  • Aquaporin 6
  • Aquaporins
  • DNA, Complementary
  • DNA-Binding Proteins
  • GATA2 Transcription Factor
  • GATA2 protein, human
  • GATA3 Transcription Factor
  • GATA3 protein, human
  • Gata2 protein, rat
  • Ion Channels
  • Trans-Activators
  • Transcription Factors

Associated data

  • GENBANK/AB000217