Induction of alternative splicing of HLA-B27 by bacterial invasion

Arthritis Rheum. 1997 Apr;40(4):694-703. doi: 10.1002/art.1780400414.


Objective: Alternative splicing of certain class I major histocompatibility complex pre-messenger RNA (pre-mRNA) is known to lead to generation of a cell-free soluble protein analog. This study was undertaken to examine whether this process occurs with HLA-B27, whether the process is modified by arthritis-causing bacteria, and whether the assembly of the soluble molecules follows the same pathway as the integral parent molecules.

Methods: Alternative splicing of pre-mRNA was analyzed by reverse transcriptase-polymerase chain reaction, and assembly of soluble HLA-B27 by immunoprecipitation followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography.

Results: There was alternative splicing of the pre-mRNA of HLA-B27. The process could be amplified by invasion with Salmonella or Yersinia bacteria. The soluble HLA-B27 was assembled in a pathway similar to that of the parent molecule.

Conclusion: The association between arthritis-causing bacteria and HLA-B27 positive cells is a complex event. Soluble HLA-B27 is a potential key player.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alternative Splicing / physiology*
  • Base Sequence
  • Cell Line / microbiology
  • Cells, Cultured
  • DNA Primers / chemistry
  • Electrophoresis, Polyacrylamide Gel
  • HLA-B27 Antigen / biosynthesis
  • HLA-B27 Antigen / genetics*
  • Humans
  • Lymphocytes / cytology
  • Lymphocytes / metabolism
  • Lymphocytes / microbiology
  • Molecular Sequence Data
  • Monocytes / cytology
  • Monocytes / metabolism
  • Monocytes / microbiology
  • Polymerase Chain Reaction
  • RNA, Bacterial / genetics
  • Salmonella typhimurium / physiology*
  • Transfection
  • Yersinia enterocolitica / physiology*


  • DNA Primers
  • HLA-B27 Antigen
  • RNA, Bacterial