Restricted growth potential of rat neural precursors as compared to mouse

Brain Res Dev Brain Res. 1997 Apr 18;99(2):253-8. doi: 10.1016/s0165-3806(97)00002-3.


Epidermal growth factor (EGF) responsive precursors isolated from the developing mouse striatum could be continually expanded in culture as free-floating spheres of cells for over 50 days. Under identical conditions, EGF-responsive precursors from the developing rat striatum could only be expanded for between 21 and 28 days, after which crisis ensued and there was a reduction in cell number at each passage. The outer regions of 28-day-old rat spheres contained a heterogeneous population of both dividing and dying cells while the cores were full of dying cells, many of which showed features consistent with apoptosis. Fibroblast growth factor-2 (FGF-2) alone did not lead to an expansion in rat striatal precursor cell number under the conditions used here. EGF combined with FGF-2 acted synergistically on cell growth, but did not prevent the final senescence and death of the rat precursors.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation / drug effects
  • Cell Division / drug effects
  • Cells, Cultured
  • Epidermal Growth Factor / pharmacology
  • Fibroblast Growth Factors / pharmacology
  • Mice
  • Mice, Inbred Strains
  • Microscopy, Electron
  • Neurons / cytology*
  • Neurons / ultrastructure
  • Rats
  • Rats, Sprague-Dawley
  • Stem Cells / cytology*
  • Stem Cells / ultrastructure


  • Fibroblast Growth Factors
  • Epidermal Growth Factor