Embryonic Lethality and Radiation Hypersensitivity Mediated by Rad51 in Mice Lacking Brca2

Nature. 1997 Apr 24;386(6627):804-10. doi: 10.1038/386804a0.

Abstract

Inherited mutations in the human BRCA2 gene cause about half of the cases of early-onset breast cancer. The embryonic expression pattern of the mouse Brca2 gene is now defined and an interaction identified of the Brca2 protein with the DNA-repair protein Rad51. Developmental arrest in Brca2-deficient embryos, their radiation sensitivity, and the association of Brca2 with Rad51 indicate that Brca2 may be an essential cofactor in the Rad51-dependent DNA repair of double-strand breaks, thereby explaining the tumour-suppressor function of Brca2.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Animals
  • BRCA2 Protein
  • Blastocyst / radiation effects
  • Brain / embryology
  • Brain / metabolism
  • Cell Division / physiology
  • Cell Survival / physiology
  • DNA-Binding Proteins / physiology*
  • Embryo, Mammalian / cytology
  • Embryonic and Fetal Development / genetics
  • Embryonic and Fetal Development / physiology
  • Embryonic and Fetal Development / radiation effects
  • Female
  • Gamma Rays
  • Gene Expression
  • Gene Targeting
  • Genes, Tumor Suppressor
  • In Situ Hybridization
  • Male
  • Mice
  • Molecular Sequence Data
  • Mutagenesis
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / physiology*
  • Polymerase Chain Reaction
  • Protein Binding
  • Rad51 Recombinase
  • Radiation Tolerance / physiology*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins
  • Stem Cells
  • Transcription Factors / genetics
  • Transcription Factors / physiology*

Substances

  • BRCA2 Protein
  • DNA-Binding Proteins
  • Neoplasm Proteins
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • RAD51 protein, S cerevisiae
  • Rad51 Recombinase
  • Rad51 protein, mouse

Associated data

  • GENBANK/U65594