Two widely used anti-DR alpha monoclonal antibodies bind to an intracellular C-terminal epitope

Hum Immunol. 1997 Mar;53(1):34-8. doi: 10.1016/s0198-8859(97)00025-6.


In this report we show that two widely-used monoclonal antibodies, TAL-1B5 and DA6.147, which react with the HLA-DR alpha chain on immunoblots, recognize the C-terminal intracellular tail of this HLA-DR subunit. We demonstrate that both MoAbs react with a synthetic peptide representing the intracellular C-terminal tail of the DR alpha chain and that mutant DR molecules lacking this part of the alpha chain lose reactivity with TAL-1B5 and DA6.147, both in Western blot analysis and in intracellular FACS staining.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Monoclonal / metabolism*
  • Binding Sites, Antibody*
  • Epitopes / immunology*
  • Epitopes / metabolism*
  • HLA-DR Antigens / genetics
  • HLA-DR Antigens / immunology*
  • HLA-DR Antigens / metabolism*
  • Humans
  • Intracellular Fluid / immunology
  • Molecular Sequence Data
  • Protein Conformation


  • Antibodies, Monoclonal
  • Epitopes
  • HLA-DR Antigens