'Semifree-floating' treatment: a simple and fast method to process consecutive sections for immunohistochemistry and neuronal tracing

J Neurosci Methods. 1997 Mar;72(1):57-63. doi: 10.1016/s0165-0270(96)00156-2.


A method is described which allows for histochemical processing of thick (50-200 microm) and consecutive sections of neural tissue, a prerequisite for many neuroanatomical studies. Two examples are given: (A) biotin-dextran-amine (BDA) tracing of neuronal connections in 50-100 microm thick vibratome sections of the adult rat brain and (B) immunohistochemical analysis of tyrosine hydroxylase-positive bulbospinal fibers in 50 microm thick cryosections of spinal cord.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biotin
  • Cerebral Cortex / chemistry
  • Cerebral Cortex / enzymology
  • Dextrans
  • Female
  • Immunohistochemistry / methods*
  • Male
  • Microscopy, Confocal
  • Microtomy / methods*
  • Rats
  • Rats, Inbred Lew
  • Spinal Cord / chemistry
  • Spinal Cord / enzymology
  • Tyrosine 3-Monooxygenase / analysis


  • Dextrans
  • Biotin
  • Tyrosine 3-Monooxygenase