Molecular cloning of murine ICA69: diabetes-prone mice recognize the human autoimmune-epitope, Tep69, conserved in splice variants from both species

Biochim Biophys Acta. 1997 Apr 12;1360(2):97-101. doi: 10.1016/s0925-4439(97)00002-1.


The islet cell antigen ICA69 is an autoimmune target in most patients with insulin-dependent diabetes. Understanding its role in diabetic autoimmunity would be facilitated by an animal model. We therefore cloned mouse ICA69. The different splice variants now identified conserve Tep69, the single T cell epitope recognized by patient T cells. We show that diabetes-prone NOD mice had Tep69-specific, autoreactive T cell repertoires and thus provide a relevant model for the study of ICA69's role in diabetic autoimmunity.

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Autoantigens / genetics*
  • Autoantigens / immunology
  • Blotting, Western
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Diabetes Mellitus, Type 1 / immunology*
  • Humans
  • Membrane Proteins / genetics
  • Membrane Proteins / immunology
  • Mice
  • Mice, Inbred NOD
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Tissue Distribution


  • Autoantigens
  • DNA, Complementary
  • ICA1 protein, human
  • Ica1 protein, mouse
  • Membrane Proteins
  • RNA, Messenger