Anti-inflammatory properties of interleukin-10 administration in hapten-induced colitis

Eur J Pharmacol. 1997 Apr 4;323(2-3):245-54. doi: 10.1016/s0014-2999(97)00017-4.


Therapeutic efficacy of interleukin-10 administration in colonic inflammation was assessed in rats. Following intracolonic instillation of 2,4,6-trinitrobenzene sulfonic acid (TNBS), subcutaneous administration of 1-1000 micrograms/kg per day interleukin-10, or a placebo (0.9% NaCl) was commenced and continued for 5 days. Interleukin-10 administered at 1, 10 and 100 micrograms/kg per day significantly reduced myeloperoxidase activity by 34, 57, and 28%, respectively, compared to the placebo-treated group, which was paralleled by an attenuation of colonic tumor necrosis factor alpha (TNF-alpha) content. In contrast, the severity of mucosal necrosis was not affected by interleukin-10 administration at the dose range used. In addition, the 10-fold elevation in nitric oxide release, 5-fold rise in colonic nitrite production and enhanced expression of inducible nitric oxide synthase, associated with TNBS colitis, was not suppressed by interleukin-10. Interleukin-10 gene expression was elevated during the first 14 days of TNBS colitis. We conclude that 5 days administration of interleukin-10 in TNBS colitis displays mild anti-inflammatory properties which were not mediated via a nitric oxide-dependent pathway, but may involve TNF-alpha.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / therapeutic use*
  • Colitis / chemically induced
  • Colitis / drug therapy*
  • Colitis / metabolism
  • Female
  • Gene Expression / drug effects
  • Interleukin-10 / genetics
  • Interleukin-10 / therapeutic use*
  • Leukocyte Count / drug effects
  • Nitric Oxide / metabolism
  • Nitric Oxide Synthase / genetics
  • Peroxidase / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Trinitrobenzenesulfonic Acid / toxicity
  • Tumor Necrosis Factor-alpha / metabolism
  • Up-Regulation


  • Anti-Inflammatory Agents
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • Nitric Oxide
  • Trinitrobenzenesulfonic Acid
  • Peroxidase
  • Nitric Oxide Synthase