Evaluation of Monoclonal Antibodies to Human Immunodeficiency Virus Type 1 Primary Isolates by Neutralization Assays: Performance Criteria for Selecting Candidate Antibodies for Clinical Trials. AIDS Clinical Trials Group Antibody Selection Working Group

J Infect Dis. 1997 May;175(5):1056-62. doi: 10.1086/516443.

Abstract

A collaborative study was organized to identify monoclonal antibodies (MAbs) that may be broadly and potently neutralizing for a panel of human immunodeficiency virus type 1 (HIV-1) low-passaged adult and pediatric primary isolates in peripheral blood mononuclear cells. Five laboratories evaluated a coded panel of seven human MAbs to HIV-1 subtype B envelope V3, CD4 binding region, gp41, and other conformationally sensitive determinants. Each laboratory measured neutralizing activity of the MAbs against the laboratory isolate HIV(MN) and a panel of 9 subtype B primary isolates. Antibodies were classified as suitable candidates for future clinical studies if they could neutralize at least half of the 9 primary isolates at a concentration of < or = 25 microg/mL for 90% viral inhibition. The study identified three MAbs that met stated performance criteria: IgG1b12, 2G12, and 2F5. These results may provide a rationale for examining the clinical efficacy, either singly or in combination, of the three MAbs.

Publication types

  • Multicenter Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acquired Immunodeficiency Syndrome / blood
  • Acquired Immunodeficiency Syndrome / immunology
  • Adult
  • Amino Acid Sequence
  • Antibodies, Monoclonal* / blood
  • Antibody Specificity
  • Antigens, CD / physiology
  • CD4 Antigens / physiology
  • Child
  • Clinical Trials as Topic / methods
  • Epitopes / analysis
  • HIV Antibodies* / blood
  • HIV Envelope Protein gp160 / chemistry
  • HIV Envelope Protein gp160 / immunology*
  • HIV Envelope Protein gp41 / chemistry
  • HIV Envelope Protein gp41 / immunology*
  • HIV Seropositivity / blood
  • HIV Seropositivity / immunology*
  • HIV-1 / classification*
  • HIV-1 / immunology
  • HIV-1 / isolation & purification*
  • Humans
  • Immunoglobulin G / blood
  • Immunoglobulin G / classification
  • Molecular Sequence Data
  • Neutralization Tests / standards*
  • Sensitivity and Specificity

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • CD4 Antigens
  • Epitopes
  • HIV Antibodies
  • HIV Envelope Protein gp160
  • HIV Envelope Protein gp41
  • Immunoglobulin G