Identification of Key Gene Products Required for Acquisition of Plasmin-Like Enzymatic Activity by Group A Streptococci

J Infect Dis. 1997 May;175(5):1115-20. doi: 10.1086/516450.

Abstract

Group A streptococci incubated in human plasma can acquire a plasmin-like enzymatic activity. This process involves at least two bacterial proteins and two human protein cofactors. In this study, the key bacterial proteins were identified by using a series of isogenic mutants of group A isolate, CS101. These studies confirm a key role for the secreted plasminogen activator, streptokinase, and identify the major surface fibrinogen-binding protein as the product of the mrp gene. The requirement for human fibrinogen and plasminogen as key cofactors was also confirmed.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins*
  • Carrier Proteins / biosynthesis
  • Carrier Proteins / chemistry
  • Carrier Proteins / metabolism*
  • Cloning, Molecular
  • DNA Primers
  • Fibrinogen / metabolism*
  • Fibrinolysin*
  • Genes, Bacterial
  • Humans
  • Membrane Proteins / biosynthesis
  • Membrane Proteins / chemistry
  • Membrane Proteins / metabolism*
  • Mutagenesis, Site-Directed
  • Plasminogen / metabolism*
  • Polymerase Chain Reaction
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / metabolism
  • Streptococcus pyogenes / enzymology*
  • Streptococcus pyogenes / genetics*
  • Streptococcus pyogenes / isolation & purification
  • Streptokinase / biosynthesis
  • Streptokinase / genetics
  • Streptokinase / metabolism*

Substances

  • Bacterial Proteins
  • Carrier Proteins
  • DNA Primers
  • Membrane Proteins
  • Mrp22 protein, Streptococcus
  • Recombinant Proteins
  • Fibrinogen
  • Plasminogen
  • Streptokinase
  • Fibrinolysin