ASP stimulates glucose transport in cultured human adipocytes

Int J Obes Relat Metab Disord. 1997 Apr;21(4):261-6. doi: 10.1038/sj.ijo.0800396.


Objective: The purpose of the present study was to examine the effect of Acylation Stimulating Protein (ASP) on glucose transport in cultured subcutaneous adipocytes.

Design and subjects: Subcutaneous adipose tissue was obtained from non-obese, healthy females (18-32 y old) undergoing mammoplasty reduction. Preadipocytes were isolated and differentiated into adipocytes.

Measurements: Following the exposure of preadipocytes and adipocytes to ASP or insulin, glucose transport was assessed as [3H] 2-deoxy glucose uptake. The measurements were normalised per total cell protein.

Results: ASP increases specific membrane glucose transport in both preadipocytes and adipocytes in a time and concentration dependent manner. Stimulation in both cell types is rapid (within minutes), reaching a maximal effect between 1 and 4 h. However, after 24 h exposure to ASP, there is a downregulation in the response. The ASP response is greater following differentiation of preadipocytes to adipocytes and is compared to that of insulin. Dose response studies demonstrated a five-fold greater sensitivity of adipocytes (half-maximal concentration of ASP on adipocytes = 0.5 microM, preadipocytes = 2.3 microM).

Conclusion: These results demonstrate that ASP not only stimulates triglyceride synthesis, but also glucose transport in differentiated human adipocytes and is consistent with a physiologically important role for ASP in postprandial energy storage.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / cytology
  • Adipocytes / drug effects
  • Adipocytes / metabolism*
  • Adult
  • Biological Transport / drug effects
  • Blood Proteins / pharmacology*
  • Breast / cytology
  • Breast / pathology
  • Cell Differentiation / drug effects
  • Cell Differentiation / physiology
  • Cells, Cultured
  • Complement C3a* / analogs & derivatives*
  • Deoxyglucose / analysis
  • Deoxyglucose / metabolism*
  • Dose-Response Relationship, Drug
  • Down-Regulation
  • Female
  • Humans
  • Insulin / pharmacology*
  • Time Factors
  • Tritium


  • Blood Proteins
  • Insulin
  • complement C3a, des-Arg-(77)-
  • Tritium
  • Complement C3a
  • Deoxyglucose