Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. May-Jul 1997;113(1-3):200-2.
doi: 10.1159/000237546.

Eosinophil Granule Proteins Are Selective Activators of Human Heart Mast Cells

Affiliations

Eosinophil Granule Proteins Are Selective Activators of Human Heart Mast Cells

V Patella et al. Int Arch Allergy Immunol. .

Abstract

Eosinophilia in humans is associated with eosinophil infiltration and cardiac localization of eosinophil granule proteins. Eosinophil cationic proteins are responsible for cardiac disease in some patients with eosinophilia. We have investigated the in vitro effect of four eosinophil granule proteins: eosinophil cationic protein (ECP), major basic protein (MBP), eosinophil-derived neurotoxin (EDN) and eosinophil peroxidase (EPO), on mast cells isolated from human cardiac tissue (HHMC). ECP and, to a lesser extent MBP (0.3-3 microM), but not EDN and EPO, stimulated the release of histamine and tryptase from HHMC. This release reaction induced by ECP and MBP was Ca(2+)- and temperature-dependent and was abolished by preincubation with anti-ECP and anti-MBP, respectively. The activation of HHMC by ECP and MBP was abolished by preincubation with 2-deoxy-D-glucose and antimycin A. These data demonstrate that some eosinophil cationic proteins, ECP and MBP, are selective activators of HHMC, thus contributing to the cardiac lesions in patients with eosinophilia.

Similar articles

See all similar articles

Cited by 4 articles

  • Bidirectional Mast Cell-Eosinophil Interactions in Inflammatory Disorders and Cancer.
    Galdiero MR, Varricchi G, Seaf M, Marone G, Levi-Schaffer F, Marone G. Galdiero MR, et al. Front Med (Lausanne). 2017 Jul 24;4:103. doi: 10.3389/fmed.2017.00103. eCollection 2017. Front Med (Lausanne). 2017. PMID: 28791287 Free PMC article. Review.
  • Eosinophils in Autoimmune Diseases.
    Diny NL, Rose NR, Čiháková D. Diny NL, et al. Front Immunol. 2017 Apr 27;8:484. doi: 10.3389/fimmu.2017.00484. eCollection 2017. Front Immunol. 2017. PMID: 28496445 Free PMC article. Review.
  • Human heart as a shock organ in anaphylaxis.
    Marone G, Genovese A, Varricchi G, Granata F. Marone G, et al. Allergo J Int. 2014;23(2):60-66. doi: 10.1007/s40629-014-0007-3. Epub 2014 Mar 19. Allergo J Int. 2014. PMID: 26120516 Free PMC article. Review.
  • Tissue remodeling in eosinophilic esophagitis.
    Cheng E, Souza RF, Spechler SJ. Cheng E, et al. Am J Physiol Gastrointest Liver Physiol. 2012 Dec 1;303(11):G1175-87. doi: 10.1152/ajpgi.00313.2012. Epub 2012 Sep 27. Am J Physiol Gastrointest Liver Physiol. 2012. PMID: 23019192 Free PMC article. Review.

LinkOut - more resources

Feedback