Mitotic activity and nuclear DNA damage of large cells in Hodgkin's disease: comparison with the expression of p53 and bcl-2 proteins and the presence of Epstein-Barr virus

Leuk Lymphoma. 1997 Mar;25(1-2):153-61. doi: 10.3109/10428199709042505.


The roles of the bcl-2 and p53 proteins in Hodgkin's disease (HD) are poorly understood. We therefore compared their detected presence in Hodgkin and Reed-Sternberg/large atypical (H-RS/LA) cells immunohistochemically with the percentages of these cells double-labeled for CD30 and DNA strand breaks (DNA fragmentation index, DFI); mitotic indices (MI); and the EBV infection status. We found a highly significant inverse correlation between the fractions per case of H-RS/LA cells expressing bcl-2/p53 proteins and the DFI of CD30+ elements. No marked effect of these two oncoproteins on MI was noticed, although these parameters and DFI of CD30+ cells were linearly related. EBV infection of H-RS/LA cells exerted only a limited effect on the parameters tested. The results of this study suggest that overexpressed bcl-2 and, to some extent, p53 proteins in H-RS/LA cells of HD primarily counteract deletion of these cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Apoptosis*
  • DNA Damage*
  • DNA, Neoplasm / genetics
  • DNA, Viral / analysis
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Herpesviridae Infections / complications
  • Herpesvirus 4, Human / genetics
  • Histocytochemistry
  • Hodgkin Disease / microbiology
  • Hodgkin Disease / pathology*
  • Humans
  • In Situ Hybridization
  • Ki-1 Antigen / analysis
  • Male
  • Middle Aged
  • Mitosis
  • Proto-Oncogene Proteins c-bcl-2 / metabolism*
  • Tumor Suppressor Protein p53 / metabolism*
  • Tumor Virus Infections / complications


  • DNA, Neoplasm
  • DNA, Viral
  • Ki-1 Antigen
  • Proto-Oncogene Proteins c-bcl-2
  • Tumor Suppressor Protein p53