Chemotaxis of macrophages by a peritoneal fluid protein in women with endometriosis

Fertil Steril. 1997 May;67(5):865-9. doi: 10.1016/s0015-0282(97)81398-2.


Objective: To expand on a preliminary study comparing the chemotactic potential of peritoneal fluid (PF) from women with and without endometriosis and to characterize this activity via immunosuppressants and a protease.

Design: Case control study.

Setting: University center.

Patient(s): Fifty-nine women with endometriosis and 44 without, undergoing laparoscopy.

Intervention(s): Collection of PF, endometriotic, ovarian, and endometrial biopsies at laparoscopy.

Main outcome measure(s): Chemotactic activity of PF was tested via an in vitro assay alone and in the presence of immunosuppressants cyclosporin A (CSA), FK506, rapamycin, and type XVII-b(S-V8) protease and in media incubated with endometriotic, ovarian, or endometrial biopsy specimens.

Result(s): The PF from women with endometriosis had significantly greater chemotactic activity (cells per well, mean +/- SD) than without endometriosis (142 +/- 39 versus 48 +/- 17). Cyclosporin A significantly inhibited the chemotactic activity of the endometriotic PF; FK506 and rapamycin did not. Incubation of media with endometriotic tissue, but not ovarian or endometrial, for > or = 7 hours displayed chemotactic activity. Protease type XVII-b(S-V8) added to endometriotic PF inhibited this chemotactic activity.

Conclusion(s): Peritoneal fluid from patients with endometriosis contains a protein chemotactic factor attracting inflammatory cells into the peritoneal cavity, possibly secreted by endometriotic implants. This chemotactic factor may be a member of the immunophilin family because of its inhibition profile.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Ascitic Fluid / chemistry*
  • Case-Control Studies
  • Chemotaxis*
  • Cyclosporine / pharmacology
  • Endometriosis / metabolism*
  • Female
  • Humans
  • Immunosuppressive Agents / pharmacology
  • Macrophages / physiology*
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology
  • Polyenes / pharmacology
  • Proteins / pharmacology*
  • Serine Endopeptidases / pharmacology
  • Sirolimus
  • Tacrolimus / pharmacology


  • Immunosuppressive Agents
  • Polyenes
  • Proteins
  • N-Formylmethionine Leucyl-Phenylalanine
  • Cyclosporine
  • Serine Endopeptidases
  • glutamyl endopeptidase
  • Sirolimus
  • Tacrolimus