Cloning and characterization of human cDNAs encoding a protein with high homology to rat intestinal development protein OCI-5

Gene. 1997 Apr 1;188(2):151-6. doi: 10.1016/s0378-1119(96)00689-0.

Abstract

We constructed a lambda complementary DNA expression library from the mitoxantrone-resistant human gastric carcinoma cell line EPG85-257RNOV. The library was screened by differential hybridization (resistant cell line against non-resistant cell variant). By this procedure we found five independent cDNA clones representing one single gene that has much higher expression in the mitoxantrone-resistant cell line EPG85-257RNOV than in the non-resistant variant EPG85-257P. One of the cDNA clones (MXR7) contains a complete open reading frame (ORF) encoding a 580-amino acid polypeptide. Amino acid and nucleotide sequence analysis revealed that this gene codes the human variant of a rat intestinal development protein OCI-5.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary
  • Gene Expression
  • Glypicans
  • Humans
  • Membrane Proteins / genetics*
  • Molecular Sequence Data
  • Neoplasm Proteins*
  • Rats
  • Tumor Cells, Cultured

Substances

  • DNA, Complementary
  • GPC3 protein, human
  • Glypicans
  • Gpc3 protein, rat
  • Membrane Proteins
  • Neoplasm Proteins

Associated data

  • GENBANK/Z37987