Insertionally inactivated and inducible recA alleles for use in Neisseria

Gene. 1997 Apr 1;188(2):215-20. doi: 10.1016/s0378-1119(96)00810-4.

Abstract

Two classes of recA mutations have been constructed for use in Neisseria gonorrhoeae: three insertionally inactivated ('knockout') mutations and three LacI-regulatable constructs that can be shifted between Rec- and Rec+ by the removal or addition of IPTG. The effects of regulating recA expression on the processes of DNA transformation, DNA repair and pilin-phase variation are described. These regulatable cassettes can also be used to control the expression of any chromosomal gene.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Drug Resistance, Microbial / genetics
  • Gene Expression Regulation, Fungal
  • Mutagenesis, Insertional*
  • Neisseria gonorrhoeae / genetics*
  • Rec A Recombinases / genetics*

Substances

  • Rec A Recombinases