Cloning and expression of cDNA encoding heart-type isoform of AMP deaminase

Gene. 1997 Apr 1;188(2):285-90. doi: 10.1016/s0378-1119(96)00818-9.

Abstract

Mouse cDNA for the heart-type (H) isoform of AMP deaminase (EC 3.5.4.6., AMPD) has been isolated and characterized. The cDNA for the Ampd gene expressed in heart is predicted to encode a peptide of 766 amino acids with a molecular mass of 88 kDa. The coding region of this cDNA was quite homologous to the human AMPD3 gene which encodes the erythrocyte-type (E) isoform of AMPD, although the H-isoform of rodent AMPD was reported to be immunologically distinct from the E-isoform of human AMPD. The non-coding region of the isolated cDNA is not homologous to human AMPD3, while rodent Ampd1 has similarity to human AMPD1 in the non-coding region as well as in the coding region. The transcripts for the cloned cDNA were expressed in heart, slow-twitch skeletal muscle and non-muscle tissues. Prokaryotic expression showed that this cDNA encodes a catalytically active enzyme which reacts to the specific antibody raised to the H-isoform of AMPD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AMP Deaminase / genetics*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary
  • Escherichia coli / metabolism
  • Gene Expression
  • Humans
  • Isoenzymes / genetics*
  • Mice
  • Molecular Sequence Data
  • Myocardium / enzymology*

Substances

  • DNA, Complementary
  • Isoenzymes
  • AMP Deaminase

Associated data

  • GENBANK/D85596