Gliostatin/platelet-derived endothelial cell growth factor as a clinical marker of rheumatoid arthritis and its regulation in fibroblast-like synoviocytes

Br J Rheumatol. 1997 Mar;36(3):315-21. doi: 10.1093/rheumatology/36.3.315.

Abstract

The objective was to assess the congruity of gliostatin/platelet-derived endothelial cell growth factor (GLS PD-ECGF) with other clinical markers of rheumatoid arthritis (RA) and to define its molecular mechanism of action in the complicated cytokine network during RA pathogenesis. Immunoassay systems were used to quantify GLS or cytokine levels in laboratory and clinical samples. Expression levels of GLS were determined by reverse transcription-polymerase chain reaction methods. The GLS levels in synovial fluid were correlated with interleukin-1 (IL-1) and IL-8. The serial data of serum GLS levels reflected well changes in the disease activity during the clinical course of four representative patients with RA. In cultured fibroblast-like synoviocytes, tumour necrosis factor-alpha (TNF-alpha), IL-1, IL-6 and IL-8 induced GLS expression. In conclusion, our results suggest that the serum GLS level, mostly derived from cytokine-stimulated synoviocytes, was a useful clinical marker of RA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arthritis, Rheumatoid / diagnosis*
  • Biomarkers / blood*
  • Blood Platelets / chemistry*
  • Endothelial Growth Factors / blood*
  • Endothelial Growth Factors / pharmacokinetics
  • Humans
  • Interleukin-1 / analysis
  • Interleukin-1 / pharmacology
  • Interleukin-6 / analysis
  • Interleukin-6 / pharmacology
  • Interleukin-8 / analysis
  • Interleukin-8 / pharmacology
  • Nerve Tissue Proteins / analysis*
  • Nerve Tissue Proteins / blood*
  • Nerve Tissue Proteins / immunology
  • Synovial Fluid / chemistry*
  • Therapeutic Equivalency
  • Thymidine Phosphorylase
  • Tumor Necrosis Factor-alpha / analysis
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • Biomarkers
  • Endothelial Growth Factors
  • Interleukin-1
  • Interleukin-6
  • Interleukin-8
  • Nerve Tissue Proteins
  • Tumor Necrosis Factor-alpha
  • Thymidine Phosphorylase