Release of Glucagon-Like peptide-1 (GLP-1) by Carbohydrates in the Perfused Rat Ileum

Acta Diabetol. 1997 Mar;34(1):18-21. doi: 10.1007/s005920050059.

Abstract

The effect of various carbohydrates on glucagon-like peptide-1 (GLP-1) release was studied in the in vivo perfused rat ileum. GLP-1 concentrations in the mesenteric venous effluent increased significantly after luminal perfusion with substrates of a sodium/glucose co-transporter (D-glucose, D-galactose, methyl-alpha D-glucoside, and 3-O-methyl-D-glucose). D-Fructose induced a sodium-independent release of GLP-1. Carbohydrates like 2-deoxy-D-glucose and N-acetyl-D-glucosamine, which are not substrates of a luminal sodium/glucose or fructose transporter, did not affect GLP-1 release. Since methyl-alpha D-glucoside is not a substrate of the basolateral glucose transport mechanism and 3-O-methyl-D-glucose is not metabolized within intestinal cells, it is concluded that intracellular metabolism of carbohydrates and intracellular removal are not essential to induce GLP-1 secretion in rats.

MeSH terms

  • 3-O-Methylglucose / pharmacology
  • Acetylglucosamine / pharmacology
  • Animals
  • Deoxyglucose / pharmacology
  • Fructose / pharmacology
  • Galactose / pharmacology
  • Glucagon-Like Peptide 1
  • Glucose / pharmacology
  • Ileum / drug effects
  • Ileum / physiology*
  • Male
  • Methylglucosides / pharmacology
  • Monosaccharide Transport Proteins / metabolism
  • Monosaccharides / pharmacology*
  • Peptides / blood
  • Peptides / metabolism*
  • Perfusion
  • Rats
  • Rats, Wistar

Substances

  • Methylglucosides
  • Monosaccharide Transport Proteins
  • Monosaccharides
  • Peptides
  • 3-O-Methylglucose
  • Fructose
  • methylglucoside
  • Glucagon-Like Peptide 1
  • Deoxyglucose
  • Glucose
  • Acetylglucosamine
  • Galactose