Abstract
Tetrahydroxybacteriohopane (1), a bacterial hopanoid, inhibited soybean 15-lipoxygenase with an IC50 of about 10 microM. After per-O-acetylation of 1 no inhibition of the 15-lipoxygenase was observed. Two other bacterial hopanoids, tetrahydroxybacteriohopane glucosamine (2) and tetrahydroxybacteriohopane ether (3), stimulated the activity of soybean 15-lipoxygenase. The activities of two other arachidonic acid-metabolizing enzymes, human 5-lipoxygenase and prostaglandin H synthase, were unaffected by 1.
MeSH terms
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Arachidonate 5-Lipoxygenase / metabolism
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Glycine max / enzymology
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Humans
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Lipoxygenase Inhibitors / isolation & purification*
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Lipoxygenase Inhibitors / pharmacology
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Prostaglandin-Endoperoxide Synthases / metabolism
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Recombinant Proteins / chemistry
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Triterpenes / isolation & purification*
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Triterpenes / pharmacology
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Zymomonas / chemistry
Substances
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Lipoxygenase Inhibitors
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Recombinant Proteins
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Triterpenes
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32,33,34,35-bacteriohopanetetrol
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Arachidonate 5-Lipoxygenase
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Prostaglandin-Endoperoxide Synthases