Vitamin E Supplementation and in Vivo Immune Response in Healthy Elderly Subjects. A Randomized Controlled Trial

JAMA. 1997 May 7;277(17):1380-6. doi: 10.1001/jama.1997.03540410058031.

Abstract

Objective: To determine whether long-term supplementation with vitamin E enhances in vivo, clinically relevant measures of cell-mediated immunity in healthy elderly subjects.

Design: Randomized, double-blind, placebo-controlled intervention study.

Setting and participants: A total of 88 free-living, healthy subjects at least 65 years of age.

Intervention: Subjects were randomly assigned to a placebo group or to groups consuming 60, 200, or 800 mg/d of vitamin E for 235 days.

Main outcome measures: Delayed-type hypersensitivity skin response (DTH); antibody response to hepatitis B, tetanus and diphtheria, and pneumococcal vaccines; and autoantibodies to DNA and thyroglobulin were assessed before and after supplementation.

Results: Supplementation with vitamin E for 4 months improved certain clinically relevant indexes of cell-mediated immunity in healthy elderly. Subjects consuming 200 mg/d of vitamin E had a 65% increase in DTH and a 6-fold increase in antibody titer to hepatitis B compared with placebo (17% and 3-fold, respectively), 60-mg/d (41% and 3-fold, respectively), and 800-mg/d (49% and 2.5-fold, respectively) groups. The 200-mg/d group also had a significant increase in antibody titer to tetanus vaccine. Subjects in the upper tertile of serum alpha-tocopherol (vitamin E) concentration (>48.4 micromol/L [2.08 mg/dL]) after supplementation had higher antibody response to hepatitis B and DTH. Vitamin E supplementation had no effect on antibody titer to diphtheria and did not affect immunoglobulin levels or levels of T and B cells. No significant effect of vitamin E supplementation on autoantibody levels was observed.

Conclusions: Our results indicate that a level of vitamin E greater than currently recommended enhances certain clinically relevant in vivo indexes of T-cell-mediated function in healthy elderly persons. No adverse effects were observed with vitamin E supplementation.

Publication types

  • Clinical Trial
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aged
  • Analysis of Variance
  • Autoantibodies / analysis
  • Cytotoxicity, Immunologic
  • Double-Blind Method
  • Female
  • Food, Fortified
  • Health Status
  • Humans
  • Hypersensitivity, Delayed / immunology
  • Immunity, Cellular / drug effects*
  • Immunoglobulins / analysis
  • Male
  • Vaccination
  • Vitamin E / administration & dosage
  • Vitamin E / pharmacology*

Substances

  • Autoantibodies
  • Immunoglobulins
  • Vitamin E