Platelet-endothelial cell adhesion molecule-1 (PECAM-1/CD31) tyrosine phosphorylation state changes during vasculogenesis in the murine conceptus

Am J Pathol. 1997 May;150(5):1523-30.


Vasculogenesis, the differentiation of mesodermal cells to angioblasts and the subsequent formation of blood islands and blood vessels by angioblasts in the conceptus, is a dynamic process modulated, in part, by cell-extracellular matrix and cell-cell interactions in the presence of a variety of growth factors and morphogens. In this report we demonstrate differential tyrosine phosphorylation of platelet-endothelial cell adhesion molecule-1 (PECAM-1) during the formation of blood islands and vessels from clusters of extraembryonic and embryonic angioblasts in the murine conceptus. In addition, we identify the phosphorylation of a particular tyrosine residue in the PECAM-1 cytoplasmic domain, Tyr686, which has the potential of mediating binding to Src homology 2 domain-containing proteins, affecting PECAM-1 cellular localization and endothelial cell migration.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibody Specificity
  • Blotting, Western
  • Embryo, Mammalian / blood supply*
  • Embryo, Mammalian / chemistry
  • Embryo, Mammalian / metabolism*
  • Immune Sera / chemistry
  • Mice
  • Neovascularization, Physiologic*
  • Phosphorylation
  • Platelet Endothelial Cell Adhesion Molecule-1 / immunology
  • Platelet Endothelial Cell Adhesion Molecule-1 / isolation & purification
  • Platelet Endothelial Cell Adhesion Molecule-1 / metabolism*
  • Precipitin Tests
  • Tyrosine / metabolism*


  • Immune Sera
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Tyrosine