Feulgen staining of intact plant tissues for confocal microscopy

Biotech Histochem. 1996 Mar;71(2):84-7. doi: 10.3109/10520299609117139.

Abstract

A method was developed to prepare plant structures for confocal laser scanning microscopy by combining Feulgen staining with pararosaniline and embedding in LR White(TM). This procedure preserves intact, delicate structures for three-dimensional imaging without loss from sectioning or squashing, and the slides can be viewed several times without serious photo-bleaching.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylic Resins
  • Coloring Agents*
  • Microscopy, Confocal / methods
  • Plants / chemistry
  • Plants / ultrastructure*
  • Rosaniline Dyes*
  • Staining and Labeling / methods*
  • Tissue Embedding

Substances

  • Acrylic Resins
  • Coloring Agents
  • Feulgen stain
  • Rosaniline Dyes
  • LR white