Plant cuticle staining with bismarck brown Y and azure B or toluidine blue O before paraffin extraction

Biotech Histochem. 1996 Mar;71(2):92-5. doi: 10.3109/10520299609117141.


Transverse paraffin sections of mature greenwood stems of rose (Rosa x hybrida) and flowering dogwood (Cornus florida L.) were stained with Bismarck brown followed by azure B or toluidine blue 0. The Bismarck brown was replaced by thiazin dye metachromasia in all structures except the cuticle which remained brown or yellow. The interface between the cuticle and exterior cell walls of the epidermis was delineated clearly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Azo Compounds*
  • Azure Stains*
  • Coloring Agents*
  • Paraffin Embedding
  • Plant Stems / chemistry
  • Plant Stems / ultrastructure
  • Plants / chemistry
  • Plants / ultrastructure*
  • Staining and Labeling / methods*
  • Tolonium Chloride*
  • Trees / chemistry
  • Trees / ultrastructure


  • Azo Compounds
  • Azure Stains
  • Coloring Agents
  • Tolonium Chloride
  • Azure B
  • Bismark Brown