Recently, avidity determination of IgG has been introduced successfully into virus serology as an additional and specific means for confirmation or exclusion of current infections. This simple and highly reproducible method can compensate for problems arising by classical serology, which include lack of detectable IgM responses during primary infections and persistent IgM responses after past infections. We show that avidity determination can be applied successfully for serological diagnosis of TBEV infection. Using the urea denaturation method, primary TBEV infections showed anti-TBEV IgG of low avidity (avidity index < 0.4), whereas sera from individuals with past infections exhibited high avidity IgG. The retrospective analysis of cases with clinical symptoms of TBEV infection in the absence of detectable anti-TBEV IgM showed that a significant number of these cases (5/45) had anti-TBEV IgG of low avidity, indicating current infection. We recommended the use of avidity determination as a method for routine TBEV serology.