A Complex Containing N-CoR, mSin3 and Histone Deacetylase Mediates Transcriptional Repression

Nature. 1997 May 1;387(6628):43-8. doi: 10.1038/387043a0.

Abstract

Transcriptional repression by nuclear receptors has been correlated to binding of the putative co-repressor, N-CoR. A complex has been identified that contains N-CoR, the Mad presumptive co-repressor mSin3, and the histone deacetylase mRPD3, and which is required for both nuclear receptor- and Mad-dependent repression, but not for repression by transcription factors of the ets-domain family. These data predict that the ligand-induced switch of heterodimeric nuclear receptors from repressor to activator functions involves the exchange of complexes containing histone deacetylases with those that have histone acetylase activity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • Binding Sites
  • Cell Line
  • DNA-Binding Proteins / physiology
  • Gene Expression Regulation*
  • HeLa Cells
  • Histone Deacetylases / physiology*
  • Humans
  • Nuclear Proteins / physiology*
  • Nuclear Receptor Co-Repressor 1
  • Protein Binding
  • Rats
  • Receptors, Cytoplasmic and Nuclear / physiology
  • Receptors, Thyroid Hormone / genetics
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Repressor Proteins / physiology*
  • Saccharomyces cerevisiae Proteins*
  • Transcription Factors / physiology*
  • Transcription, Genetic
  • Transfection

Substances

  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • DNA-Binding Proteins
  • MXD1 protein, human
  • NCOR1 protein, human
  • Ncor1 protein, rat
  • Nuclear Proteins
  • Nuclear Receptor Co-Repressor 1
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Thyroid Hormone
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • SIN3 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Histone Deacetylases