Expression and immunogenicity in rats of recombinant adenovirus 5 DNA plasmids and vaccinia virus containing the HTLV-I env gene

Int J Cancer. 1997 Apr 10;71(2):300-7. doi: 10.1002/(sici)1097-0215(19970410)71:2<300::aid-ijc27>;2-j.


The complete human T-cell leukemia virus type I (HTLV-I) env gene was inserted into an expression cassette containing the adenovirus 5 major late promoter (Ad5-MLP). Recombinant Ad5-HTLV-I-env was obtained by homologous recombination in 293 cells simultaneously transfected by the expression cassette and the genomic DNA of Ad5. In vitro expression of the HTLV-I-env gene in the recombinant vector was detected by immunofluorescence and Western blotting. Functional expression of HTLV-I-env was confirmed by syncitium formation specifically in HeLa cells infected with Ad5-HTLV-I-env. Two immunization regimens against HTLV-I were tested in WKY and Fischer F-344 rats. The first involved WKY rats primed with Ad5-HTLV-I-env or naked DNA plasmids containing the HTLV-I-env gene and boosted with Ad5 containing the HTLV-I-env gp46 gene or with baculovirus-derived recombinant gp46. No antibody against HTLV-I was detected, while HTLV-I-specific cytotoxic T lymphocytes were recovered from all immunized groups but not from controls. The second approach involved Fischer F-344 rats primed and boosted with recombinant vaccinia virus containing the HTLV-I-env gene. Such rats developed antibodies against the HTLV-I env gp21 and gp46 (non-neutralizing). After challenge with human HTLV-I-producing cells (MT-2), both immunization regimens were found to induce partial protection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics*
  • Animals
  • Blotting, Western
  • Cytotoxicity, Immunologic
  • DNA Primers / chemistry
  • DNA, Viral / genetics*
  • Fluorescent Antibody Technique, Indirect
  • Gene Expression Regulation, Viral* / genetics
  • Gene Expression Regulation, Viral* / immunology
  • Genes, env* / genetics
  • Genes, env* / immunology
  • HTLV-I Antibodies / analysis
  • HeLa Cells / virology
  • Human T-lymphotropic virus 1 / genetics*
  • Human T-lymphotropic virus 1 / immunology*
  • Humans
  • Plasmids / genetics
  • Rats
  • Rats, Inbred F344
  • Rats, Inbred WKY
  • T-Lymphocytes, Cytotoxic / immunology
  • Vaccinia virus / genetics*
  • Viral Envelope Proteins / immunology


  • DNA Primers
  • DNA, Viral
  • HTLV-I Antibodies
  • Viral Envelope Proteins