Cell mediated immunity induced in mice by HPV 16 L1 virus-like particles

Microb Pathog. 1997 Apr;22(4):219-25. doi: 10.1006/mpat.1996.0113.

Abstract

Recombinant human papillomavirus (HPV) type 16 L1 virus-like particles (VLPs) expressed in the baculovirus system were used to investigate the cellular immune response to human papillomavirus type 16. The cell-mediated immune response was evaluated through immunization of mice with HPV 16 L1 virus-like particles using a lymphoproliferation assay and cytokine production and cytometric analysis of lymphocyte subsets. A significant proliferative response was observed which was associated with secretion of both interferon-gamma and interleukin-2. FACS analysis of splenic lymphocytes revealed that CD8+ T-cells were increased in the immunized mice. These results demonstrate that HPV 16 L1 VLPs induce a T-cell response characterized by a Th1 profile and confirm that the HPV 16 VLP is a reasonable candidate for vaccine development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / cytology
  • CD4-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / cytology
  • CD8-Positive T-Lymphocytes / immunology*
  • Capsid Proteins*
  • Cell Division
  • Cells, Cultured
  • Humans
  • Immunity, Cellular
  • Interferon-gamma / analysis
  • Interleukin-2 / analysis
  • Interleukin-4 / analysis
  • Interleukin-5 / analysis
  • Mice
  • Oncogene Proteins, Viral / genetics
  • Oncogene Proteins, Viral / immunology*
  • Papillomaviridae / immunology*
  • Phenotype
  • Rats
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology
  • Spleen / cytology
  • Spleen / immunology*

Substances

  • Capsid Proteins
  • Interleukin-2
  • Interleukin-5
  • Oncogene Proteins, Viral
  • Recombinant Fusion Proteins
  • Interleukin-4
  • L1 protein, Human papillomavirus type 16
  • Interferon-gamma