Highly attenuated modified vaccinia virus Ankara (MVA) as an effective recombinant vector: a murine tumor model

Vaccine. 1997 Mar;15(4):387-94. doi: 10.1016/s0264-410x(96)00195-8.


Modified vaccinia virus Ankara (MVA), a highly attenuated strain of vaccinia virus (VV) that is unable to replicate in most mammalian cells, was evaluated as an expression vector for a model tumor associated antigen (TAA) and as a potential anti-cancer vaccine. We employed an experimental murine model in which an adenocarcinoma tumor line, CT26.CL25, was stably transfected with a model TAA, beta-galactosidase (beta-gal). Mice injected intramuscularly with a recombinant MVA (rMVA) expressing beta-gal (MVA-LZ), were protected from a lethal intravenous (i.v.) challenge with CT26.CL25. In addition, splenocytes from mice primed with MVA-LZ were therapeutically effective upon adoptive transfer to mice bearing pulmonary metastases of the CT26.CL25 tumor established 3 days earlier. Most importantly, i.v. inoculation with MVA-LZ resulted in significantly prolonged survival of mice bearing three day old pulmonary metastases. This prolonged survival compared favorably to mice treated with a replication competent recombinant VV expressing beta-gal. These findings indicate that rMVA is an efficacious alternative to the more commonly used replication competent VV for the development of new recombinant anti-cancer vaccines.

MeSH terms

  • Adenocarcinoma / mortality
  • Adenocarcinoma / prevention & control
  • Adenocarcinoma / secondary
  • Animals
  • Antigens, Neoplasm / immunology*
  • Antigens, Tumor-Associated, Carbohydrate / biosynthesis
  • Antigens, Tumor-Associated, Carbohydrate / immunology
  • Cancer Vaccines / immunology*
  • Colonic Neoplasms / immunology
  • Cytokines / biosynthesis
  • Disease Models, Animal
  • Female
  • Genetic Vectors / immunology*
  • Immunization, Secondary
  • Immunotherapy, Adoptive / methods
  • Lung Neoplasms / mortality
  • Lung Neoplasms / prevention & control
  • Lung Neoplasms / secondary
  • Mice
  • Mice, Inbred BALB C
  • Spleen / immunology
  • Spleen / metabolism
  • Spleen / transplantation
  • Tumor Cells, Cultured
  • Vaccines, Attenuated / immunology
  • Vaccines, Synthetic / immunology
  • Vaccinia virus / immunology*
  • Vaccinia virus / physiology
  • Virus Replication
  • beta-Galactosidase / biosynthesis


  • Antigens, Neoplasm
  • Antigens, Tumor-Associated, Carbohydrate
  • Cancer Vaccines
  • Cytokines
  • Vaccines, Attenuated
  • Vaccines, Synthetic
  • beta-Galactosidase