Activation-induced expression of human programmed death-1 gene in T-lymphocytes

Exp Cell Res. 1997 Apr 10;232(1):25-8. doi: 10.1006/excr.1997.3493.


The Programmed Death-1 (PD-1) gene is a member of the immunoglobulin superfamily of genes. Murine PD-1 mRNA expression has been shown to correlate with activation-induced apoptosis in a mouse T-cell hybridoma cell line and in murine thymocytes. Here we report that expression of the human homolog, hPD-1, seems to correlate with activation of T lymphocytes rather than apoptosis. We observed a time-dependent upregulation of hPD-1 mRNA and protein levels in Jurkat cells during phorbol ester (12-O-tetradecanoylphorbol 13-acetate, TPA)-induced differentiation. Human PD-1 protein was also induced during lectin-stimulated activation of human peripheral blood mononuclear cells. Additionally, TPA stimulation of Jurkat cells induces tyrosine phosphorylation of hPD-1, putatively on its cytoplasmic tail signal transduction motif. These data suggest a role for hPD-1 during activation and differentiation of T-lymphocytes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, CD
  • Antigens, Surface / biosynthesis*
  • Apoptosis Regulatory Proteins
  • Cell Differentiation
  • Humans
  • Jurkat Cells
  • Lymphocyte Activation*
  • Phosphorylation
  • Programmed Cell Death 1 Receptor
  • Signal Transduction
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / metabolism*
  • Tetradecanoylphorbol Acetate / pharmacology
  • Tyrosine / metabolism
  • Up-Regulation


  • Antigens, CD
  • Antigens, Surface
  • Apoptosis Regulatory Proteins
  • PDCD1 protein, human
  • Pdcd1 protein, mouse
  • Programmed Cell Death 1 Receptor
  • Tyrosine
  • Tetradecanoylphorbol Acetate