Comparison of three different methods for radiolabelling human activated T lymphocytes

Eur J Nucl Med. 1997 May;24(5):497-504. doi: 10.1007/BF01267680.


One approach in the treatment of ovarian cancer patients involves the infusion of autologous T lymphocytes coupled with a bispecific monoclonal antibody MOv18/anti-CD3 (biMAb OC/TR), which recognizes a 38-kDa glycoprotein expressed on ovarian carcinomas and the CD3 T cell receptor. However, little is known about the in vivo biodistribution of injected activated lymphocytes, information that could be obtained by scintigraphic imaging of radiolabelled T cells in order to visualize the migratory pattern. We compared the efficiency, stability and toxicity of technetium-99m hexamethylpropylene amine oxime (HMPAO), indium-111 oxine and fluorine-18 2-fluoro-2-deoxy-d-glucose (FDG) in radiolabelling activated lymphocytes targeted with biMAb OC/TR. The mean labelling efficiencies of 111In-oxine and 18F-FDG using 2.5x10(8) lymphocytes (68% and 64%, respectively) were more than twice that of 99mTc-HMPAO (31%). Retention of the radionuclide in the cell was highest in the case of 111In-oxine labelling (less than 25% of the initial cell-bound activity released after 240 min, as compared with 44% of the 99mTc label in the same period and 45% of 18F radionuclide released after 150 min). None of the three radiolabelling reagents induced any significant alteration in cell viability or immunophenotype. However, both 111In-oxine and 18F-FDG induced a loss of cytotoxic activity of lymphocytes against the ovarian carcinoma cell line IGROV1, and all three radiolabelling reagents caused a significant reduction in the proliferative ability of labelled lymphocytes compared to controls, with cell death occurring after 8-9 days. Radiolabelling with the more stable 111In-oxine reagent using a higher number of lymphocytes (1.4x10(9)) but the same total activity (around 55.5 MBq) resulted in improved labelled T cell viability and proliferative ability, although the mean labelling efficiency decreased (35.8%). Together the data suggest that 111In-oxine at low activity per cell is the most appropriate reagent for radiolabelling activated retargeted T lymphocytes useful for in vivo biodistribution studies.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Death
  • Cell Survival
  • Cells, Cultured
  • Deoxyglucose / analogs & derivatives*
  • Female
  • Fluorine Radioisotopes*
  • Fluorodeoxyglucose F18
  • Humans
  • Indium Radioisotopes*
  • Isotope Labeling / methods*
  • Lymphocyte Activation
  • Organometallic Compounds*
  • Organotechnetium Compounds*
  • Ovarian Neoplasms / immunology
  • Oximes*
  • Oxyquinoline / analogs & derivatives*
  • T-Lymphocytes / immunology
  • T-Lymphocytes / physiology*
  • Technetium Tc 99m Exametazime
  • Tumor Cells, Cultured


  • Fluorine Radioisotopes
  • Indium Radioisotopes
  • Organometallic Compounds
  • Organotechnetium Compounds
  • Oximes
  • Fluorodeoxyglucose F18
  • indium oxine
  • Technetium Tc 99m Exametazime
  • Oxyquinoline
  • Deoxyglucose