Stereospecific oxidation of (R)- and (S)-1-indanol by naphthalene dioxygenase from Pseudomonas sp. strain NCIB 9816-4

Appl Environ Microbiol. 1997 May;63(5):2067-70. doi: 10.1128/aem.63.5.2067-2070.1997.

Abstract

A recombinant Escherichia coli strain which expresses naphthalene dioxygenase (NDO) from Pseudomonas sp. strain NCIB 9816-4 oxidized (S)-1-indanol to trans-(1S,3S)-indan-1,3-diol (95.5%) and (R)-3-hydroxy-1-indanone (4.5%). The same cells oxidized (R)-1-indanol to cis-1,3-indandiol (71%), (R)-3-hydroxy-1-indanone (18.2%), and cis-1,2,3-indantriol (10.8%). Purified NDO oxidized (S)-1-indenol to both syn- and anti-2,3-dihydroxy-1-indanol.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Dioxygenases
  • Escherichia coli / genetics
  • Gene Expression Regulation, Bacterial
  • Indans / chemistry
  • Indans / metabolism*
  • Isomerism
  • Molecular Structure
  • Multienzyme Complexes / genetics*
  • Multienzyme Complexes / metabolism*
  • Oxidation-Reduction
  • Oxygenases / genetics*
  • Oxygenases / metabolism*
  • Pseudomonas / genetics*
  • Recombinant Proteins / metabolism
  • Recombination, Genetic

Substances

  • Indans
  • Multienzyme Complexes
  • Recombinant Proteins
  • 1-indanol
  • Oxygenases
  • Dioxygenases
  • naphthalene dioxygenase