Immunolocalization of proton-ATPase in the gills of the elasmobranch, Squalus acanthias

J Exp Zool. 1997 Jun 1;278(2):78-86. doi: 10.1002/(sici)1097-010x(19970601)278:2<78::aid-jez2>3.0.co;2-1.

Abstract

Proton-ATPase was localized to mitochondria-rich cells in the interlamellar region of the gills of the elasmobranch, Squalus acanthias. Localization was accomplished using a polyclonal antibody specific for the 70 kDa subunit of the (V-type) proton-ATPase as confirmed by Western blot analysis. In addition, significant levels of N-ethymaleimide sensitive ATPase activity (0.116 +/- 0.026 mumol Pi.mg-1 protein.h-1) were also measured in crude gill membrane preparations. These data provide, for the first time, direct evidence of the localization of elements possibly involved in branchial acid-base (or ionic) regulation in elasmobranchs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Animals
  • Blotting, Western
  • Dogfish / metabolism*
  • Fluorescent Antibody Technique, Indirect
  • Gills / cytology
  • Gills / enzymology*
  • Microscopy, Electron
  • Microscopy, Phase-Contrast
  • Mitochondria / ultrastructure
  • Proton Pumps / metabolism*
  • Proton-Translocating ATPases / analysis
  • Proton-Translocating ATPases / metabolism*

Substances

  • Proton Pumps
  • Adenosine Triphosphate
  • Proton-Translocating ATPases