A novel pair of immunoglobulin-like receptors expressed by B cells and myeloid cells

Proc Natl Acad Sci U S A. 1997 May 13;94(10):5261-6. doi: 10.1073/pnas.94.10.5261.


An Fcalpha receptor probe of human origin was used to identify novel members of the Ig gene superfamily in mice. Paired Ig-like receptors, named PIR-A and PIR-B, are predicted from sequence analysis of the cDNAs isolated from a mouse splenic library. Both type I transmembrane proteins possess similar ectodomains with six Ig-like loops, but have different transmembrane and cytoplasmic regions. The predicted PIR-A protein has a short cytoplasmic tail and a charged Arg residue in the transmembrane region that, by analogy with the FcalphaR relative, suggests the potential for association with an additional transmembrane protein to form a signal transducing unit. In contrast, the PIR-B protein has an uncharged transmembrane region and a long cytoplasmic tail containing four potential immunoreceptor tyrosine-based inhibitory motifs. These features are shared by the related killer inhibitory receptors. PIR-A proteins appear to be highly variable, in that predicted peptide sequences differ for seven randomly selected PIR-A clones, whereas PIR-B cDNA clones are invariant. Southern blot analysis with PIR-B and PIR-A-specific probes suggests only one PIR-B gene and multiple PIR-A genes. The PIR-A and PIR-B genes are expressed in B lymphocytes and myeloid lineage cells, wherein both are expressed simultaneously. The characteristics of the highly-conserved PIR-A and PIR-B genes and their coordinate cellular expression suggest a potential regulatory role in humoral, inflammatory, and allergic responses.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • B-Lymphocytes / immunology*
  • Chromosome Mapping*
  • Chromosomes, Human, Pair 19*
  • DNA / metabolism
  • DNA, Complementary
  • Gene Expression
  • Gene Library
  • Genetic Markers
  • Genetic Variation
  • Hematopoietic Stem Cells / immunology*
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Multigene Family
  • Organ Specificity
  • Polymerase Chain Reaction
  • Receptors, Immunologic / biosynthesis*
  • Receptors, Immunologic / chemistry
  • Receptors, Immunologic / genetics
  • Sequence Homology, Amino Acid
  • Spleen / immunology


  • DNA, Complementary
  • Genetic Markers
  • Pira1 protein, mouse
  • Pira2 protein, mouse
  • Pira3 protein, mouse
  • Pira4 protein, mouse
  • Pira5 protein, mouse
  • Pira6 protein, mouse
  • Pira7 protein, mouse
  • Pirb protein, mouse
  • Receptors, Immunologic
  • DNA

Associated data

  • GENBANK/AF038149
  • GENBANK/U96682
  • GENBANK/U96683
  • GENBANK/U96684
  • GENBANK/U96685
  • GENBANK/U96686
  • GENBANK/U96687
  • GENBANK/U96688
  • GENBANK/U96689
  • GENBANK/U96690
  • GENBANK/U96691
  • GENBANK/U96692
  • GENBANK/U96693