Binding sequence of STAT4: STAT4 complex recognizes the IFN-gamma activation site (GAS)-like sequence (T/A)TTCC(C/G)GGAA(T/A)

Biochem Biophys Res Commun. 1997 Apr 7;233(1):126-32. doi: 10.1006/bbrc.1997.6415.


Studies of transcriptional activation by interferons and various cytokines have led to the identification of a family of proteins that serve as signal transducers and activators of transcription (STAT). STAT4 is phosphorylated following interleukin (IL)-12 stimulation and is required for IL-12 signal transduction. By immunoprecipitation and PCR amplification, a specific consensus sequence for DNA binding of the STAT4 complex was determined. The binding sequence of the STAT4 complex, (T/A)TTCC(C/G)GGAA(T/A), proved to be palindromic and similar to the IFN-gamma activated site (GAS)-like sequence. The first (T/A) and last (T/A) sites of the consensus sequence were critical for the binding affinity of the STAT4 complex.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • COS Cells
  • Consensus Sequence
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / metabolism*
  • Interferon-gamma / metabolism*
  • Interleukin-12 / pharmacology
  • Molecular Sequence Data
  • Mutagenesis
  • Oligonucleotide Probes
  • Polymerase Chain Reaction
  • Precipitin Tests
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • STAT4 Transcription Factor
  • Trans-Activators / chemistry
  • Trans-Activators / metabolism*


  • DNA-Binding Proteins
  • Oligonucleotide Probes
  • Recombinant Proteins
  • STAT4 Transcription Factor
  • Trans-Activators
  • Interleukin-12
  • Interferon-gamma