Abstract
A novel G protein-coupled receptor was isolated from a cDNA derived from the cell line NH15-CA2 and a cDNA library from adult mouse brain using a PCR cloning strategy. The amino acid sequence of the candidate receptor DEZ showed homology to neuropeptide and chemoattractant receptors. Highest overall homology was found with the orphan receptor GPR-1 (65%), the angiotensin II receptor (62%), and the C5a anaphylatoxin receptor (60%). Northern blot analysis of dez revealed a predominant 2.6 kb mRNA species in NH15-CA2 cells. In situ hybridization experiments showed that dez is differentially regulated during development, with a prominent expression in developing osseous and cartilaginous tissue. It was also detectable in the adult parathyroid glands, hinting at a possible function in bone metabolism.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Bone Development*
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Bone and Bones / embryology
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Bone and Bones / metabolism
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Brain / embryology
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Brain / metabolism
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Cloning, Molecular
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GTP-Binding Proteins / metabolism*
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Humans
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Mice
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Molecular Sequence Data
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N-Formylmethionine Leucyl-Phenylalanine / metabolism
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Receptors, Formyl Peptide
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Receptors, Immunologic / biosynthesis
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Receptors, Immunologic / chemistry*
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Receptors, Neuropeptide / biosynthesis
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Receptors, Neuropeptide / chemistry*
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Receptors, Peptide / biosynthesis
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Receptors, Peptide / chemistry*
Substances
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Receptors, Formyl Peptide
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Receptors, Immunologic
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Receptors, Neuropeptide
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Receptors, Peptide
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N-Formylmethionine Leucyl-Phenylalanine
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GTP-Binding Proteins
Associated data
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GENBANK/U79526
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GENBANK/U79527