A novel G protein-coupled receptor with homology to neuropeptide and chemoattractant receptors expressed during bone development

Biochem Biophys Res Commun. 1997 Apr 17;233(2):336-42. doi: 10.1006/bbrc.1997.6455.


A novel G protein-coupled receptor was isolated from a cDNA derived from the cell line NH15-CA2 and a cDNA library from adult mouse brain using a PCR cloning strategy. The amino acid sequence of the candidate receptor DEZ showed homology to neuropeptide and chemoattractant receptors. Highest overall homology was found with the orphan receptor GPR-1 (65%), the angiotensin II receptor (62%), and the C5a anaphylatoxin receptor (60%). Northern blot analysis of dez revealed a predominant 2.6 kb mRNA species in NH15-CA2 cells. In situ hybridization experiments showed that dez is differentially regulated during development, with a prominent expression in developing osseous and cartilaginous tissue. It was also detectable in the adult parathyroid glands, hinting at a possible function in bone metabolism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Bone Development*
  • Bone and Bones / embryology
  • Bone and Bones / metabolism
  • Brain / embryology
  • Brain / metabolism
  • Cloning, Molecular
  • GTP-Binding Proteins / metabolism*
  • Humans
  • Mice
  • Molecular Sequence Data
  • N-Formylmethionine Leucyl-Phenylalanine / metabolism
  • Receptors, Formyl Peptide
  • Receptors, Immunologic / biosynthesis
  • Receptors, Immunologic / chemistry*
  • Receptors, Neuropeptide / biosynthesis
  • Receptors, Neuropeptide / chemistry*
  • Receptors, Peptide / biosynthesis
  • Receptors, Peptide / chemistry*


  • Receptors, Formyl Peptide
  • Receptors, Immunologic
  • Receptors, Neuropeptide
  • Receptors, Peptide
  • N-Formylmethionine Leucyl-Phenylalanine
  • GTP-Binding Proteins

Associated data

  • GENBANK/U79526
  • GENBANK/U79527