Differential expression of tenascin-C and tenascin-X in human astrocytomas

Acta Neuropathol. 1997 May;93(5):431-7. doi: 10.1007/s004010050636.


Tenascins (TNs) are a family of extracellular matrix glycoproteins. The first member of this family to be recognized, tenascin-C (TN-C), is known to be expressed in various tumors including human astrocytomas. Tenascin-X (TN-X) is the latest member of the TN family to be reported, and its expression in tumor tissues has not yet been examined. In this study, we found expression of TN-X in glioma cell lines and human astrocytomas by immunoblot analysis using anti-mouse TN-X antibodies. We also examined the expression of TN-C and TN-X immunohistochemically in a series of 32 human astrocytomas and tissue from 5 normal brains. Expression of TN-X was up-regulated to a higher degree in low-grade astrocytomas than in high-grade astrocytomas. TN-X was mainly localized in the perivascular stroma around tumor vessels, and weakly expressed in the intercellular spaces among tumor cells. In contrast, TN-C was more strongly expressed in the intercellular spaces and in tumor vessels in high-grade astrocytomas (anaplastic astrocytomas and glioblastomas) than in low-grade astrocytomas. In the tissues expressing both TNs, the distribution of TN-X was often reciprocal to that of TN-C. These findings indicate that the expression of TN-C and TN-X in astrocytomas is different, and that these glycoproteins could be involved in neovascularization in different manners.

MeSH terms

  • Animals
  • Astrocytoma / metabolism*
  • Brain Neoplasms / metabolism*
  • Cell Division / physiology
  • Cell Line
  • Humans
  • Immunoblotting
  • Immunohistochemistry
  • Mice
  • Tenascin / biosynthesis*
  • Up-Regulation / physiology


  • Tenascin
  • tenascin X