The amino acid sequence of mitogenic lectin-B from the roots of pokeweed (Phytolacca americana)

Biosci Biotechnol Biochem. 1997 Apr;61(4):690-8. doi: 10.1271/bbb.61.690.


The complete amino acid sequence of pokeweed lectin-B (PL-B) has been analyzed by first sequencing seven lysylendopeptidase peptides derived from the reduced and S-pyridylethylated PL-B and then connecting them by analyzing the arginylendopeptidase peptides from the reduced and S-carboxymethylated PL-B. PL-B consists of 295 amino acid residues and two oligosaccharides linked to Asn96 and Asn139, and has a molecular mass of 34,493 Da. PL-B is composed of seven repetitive chitin-binding domains having 48-79% sequence homology with each other. Twelve amino acid residues including eight cysteine residues in these domains are absolutely conserved in all other chitin-binding domains of plant lectins and class I chitinases. Also, it was strongly suggested that the extremely high hemagglutinating and mitogenic activities of PL-B may be ascribed to its seven-domain structure.

MeSH terms

  • Amino Acid Sequence
  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Lectins / chemistry*
  • Molecular Sequence Data
  • Peptide Fragments / chemistry
  • Peptide Fragments / isolation & purification
  • Plant Lectins
  • Plant Roots / chemistry*
  • Pokeweed Mitogens*
  • Sequence Homology, Amino Acid


  • Lectins
  • Peptide Fragments
  • Plant Lectins
  • Pokeweed Mitogens
  • lectin B, Phytolacca americana