Low doses of insulin-like growth factor-I improve nitrogen retention and food efficiency in rats with early cirrhosis

J Hepatol. 1997 Jan;26(1):191-202. doi: 10.1016/s0168-8278(97)80026-8.


Background/aims: In order to ascertain whether malnutrition is an early-onset feature of liver cirrhosis and whether the anabolic hormone insulin-like growth factor I (IGF-I) could be useful in the treatment of this complication, we analyzed the nutritional alterations present in rats with early-stage liver cirrhosis and the effects of IGF-I on nutritional parameters in these animals.

Methods: After a 24 h fast, a 15N-enriched diet was administered for 5 days to normal control rats and to cirrhotic rats receiving subcutaneous injections of vehicle (Group 1) or IGF-I, 2 micrograms.100 g bw-1.day-1, (Group 2) during the 5 experimental days. 15N, a stable N isotope, was measured in biological samples by mass spectrometry.

Results: Compared with control rats, Group 1 animals showed significant reductions in N intake and food efficiency (p < 0.05, both). In addition, the weight of the gastrocnemius muscle, its total N content and the dietary N content of this muscle were significantly lower in Group 1 than in control animals (p < 0.05, all). In rats from Group 2, mean values of N intake, food efficiency, gastrocnemius N content and the amount of dietary N incorporated into this muscle were similar to those in control rats, and (with the exception of gastrocnemius N total content) significantly higher than those in non-treated cirrhotic rats (p < 0.05, all).

Conclusions: A variety of nutritional disturbances were detected in rats from the early stages of liver cirrhosis. Low doses of IGF-I were found to reverse most of these changes. These results stimulate further studies to determine whether IGF-I might be useful in the correction of the malnutrition present in patients with liver cirrhosis.

MeSH terms

  • Acute Disease
  • Animals
  • Body Weight / physiology
  • Diet
  • Dose-Response Relationship, Drug
  • Feces / chemistry
  • Hydroxyproline / metabolism
  • Insulin-Like Growth Factor I / therapeutic use*
  • Linear Models
  • Liver / metabolism
  • Liver / pathology
  • Liver Cirrhosis, Experimental / complications
  • Liver Cirrhosis, Experimental / drug therapy*
  • Liver Cirrhosis, Experimental / metabolism
  • Liver Cirrhosis, Experimental / pathology
  • Male
  • Nitrogen / metabolism*
  • Nitrogen Isotopes
  • Nutrition Disorders / complications*
  • Nutrition Disorders / metabolism
  • Nutrition Disorders / pathology
  • Rats
  • Rats, Wistar


  • Nitrogen Isotopes
  • Insulin-Like Growth Factor I
  • Nitrogen
  • Hydroxyproline