Ultrastructural evidence for a direct pathway from the pontine micturition center to the parasympathetic preganglionic motoneurons of the bladder of the cat

Abstract

Light microscopy tracing studies have provided evidence that the pontine micturition center (PMC) projects to the area of the intermediolateral cell column of the sacral spinal cord. Although this region contains parasympathetic preganglionic motoneurons of the bladder and colon, it also contains many local interneurons and neurons projecting to supraspinal levels. The present study demonstrates that neurons in the PMC indeed project to preganglionic bladder motoneurons. Wheat germ agglutinin horseradish peroxidase was injected in the PMC and cholera toxin B subunit was injected in the bladder wall. Many anterogradely labeled fibers from the PMC were found to terminate on somata and dendrites of the retrogradely labeled preganglionic bladder motoneurons. The terminals were filled with many round vesicles and possessed an asymmetric synaptic cleft, suggesting an excitatory function.