Enzymology of the degradation of (di)chlorobenzenes by Xanthobacter flavus 14p1

Arch Microbiol. 1997 Jun;167(6):384-91. doi: 10.1007/s002030050459.


Xanthobacter flavus 14p1 used 1,4-dichlorobenzene as the sole source of carbon and energy but did not grow on other (chloro)aromatic compounds. 1,4-Dichlorobenzene was attacked by a chlorobenzene dioxygenase, and the intermediate chlorocatechol was metabolized by the modified ortho pathway. All enzymes necessary to convert 1, 4-dichlorobenzene to 3-oxoadipate showed a low substrate specificity and also accepted the respective intermediates of chlorobenzene or 1, 3-dichlorobenzene degradation. Of the three compounds chlorobenzene, 1,4-dichlorobenzene, and 1,3-dichlorobenzene, the latter was the most toxic for X. flavus 14p1. Furthermore, 1,3-dichlorobenzene did not induce chlorocatechol 1,2-dioxygenase activity of the organism. Chlorobenzene, however, induced chlorocatechol 1,2-dioxygenase, dienelactone hydrolase, and maleylacetate reductase activities. As demonstrated by chloride release, also chlorobenzene dioxygenase, chlorobenzene cis-dihydrodiol dehydrogenase, and chloromuconate cycloisomerase activities were present in chlorobenzene-induced cells, but chlorobenzene failed to support growth. Presumably a toxic compound was formed from one of the intermediates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biodegradation, Environmental
  • Carboxylic Ester Hydrolases / metabolism
  • Chlorides / metabolism
  • Chlorobenzenes / chemistry
  • Chlorobenzenes / metabolism*
  • Dioxygenases*
  • Environmental Pollutants / metabolism
  • Gram-Negative Aerobic Bacteria / enzymology
  • Gram-Negative Aerobic Bacteria / growth & development
  • Gram-Negative Aerobic Bacteria / metabolism*
  • Intramolecular Lyases*
  • Isomerases / metabolism
  • Oxidoreductases / metabolism
  • Oxidoreductases Acting on CH-CH Group Donors*
  • Oxygenases / metabolism
  • Substrate Specificity


  • Chlorides
  • Chlorobenzenes
  • Environmental Pollutants
  • 1,3-dichlorobenzene
  • 4-dichlorobenzene
  • Oxidoreductases
  • Oxygenases
  • Dioxygenases
  • chlorobenzene dioxygenase
  • Oxidoreductases Acting on CH-CH Group Donors
  • chlorobenzene dehydrogenase
  • maleylacetate reductase
  • Carboxylic Ester Hydrolases
  • carboxymethylenebutenolidase
  • Isomerases
  • Intramolecular Lyases
  • chloromuconate cycloisomerase